27th Politzer Meeting Poster Submissions - Sensorineural hearing loss

Abstract Title: Tanakan in the Treatment of Tinnitus
Main Organisation Work/Study Carried Out on Behalf of: University Clinic of ENT, Skopje, Republic of Macedonia
Main Authors:

  • Elena Bogeska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Marina Davceva-Cakar, Professor, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Ilija Filipce, Professor, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Irena Duma- Vasovska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Valentina Ivanovska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia

Main Contact E-mail: elenabogeska@yahoo.com
Abstract:
Objectives:  The aim of this study is to contribute the approach in treating tinnitus suffering patients. We investigated the effects of Tanakan used to treat five different groups of patients according to their diagnosis and which complained of tinnitus. 212 patients with unilateral or bilateral tinnitus: 33 with normal hearing, 12 diagnosed as trauma acustica acuta, 29 cases with Mo. Ménière, 41 cases with SSNHL, 34 cases with presbyacusis and 63 diagnosed as SNHL of unknown etiology. 
Methods: TLA, timpanometry with stapes reflex and TeOAEs to determine the hearing status. The effects of Tanakan were graduated as: none effect, improvement and complete disappearance in the period of tree months of treatment.
Results: This drug showed worst effects in patients who had normal hearing level and tinnitus, and in spite of this, patients who had acute acoustic trauma, SSNHL and Mo.Ménière had great improvement or disappearances of tinnitus.
Conclusion: These results can be explained by the vasoregulatory, haemorreorogical and neurothropical effect of Tanakan.

Abstract Title: Proteomic Analysis of Blood in Patients with Sudden Sensorineural Hearing Loss
Main Organisation Work/Study Carried Out on Behalf of: Departemnt of Otolaryngology, Kaohsiung Medical University Hospital, Taiwan
Main Authors:

  • Chen-Yu Chien, Medical Doctor, Department of Otolaryngology, Kaohsiung Medical University Hospital, Taiwan, Kaohsiung City, Taiwan

Additional Authors:

  • Kuo-Feng Hung, Student of Graduate Institute, Department of Beauty Science, Meiho Institute of Technology, Taiwan, Pingtung County, Taiwan
  • Yu-Jen Wu, Assistant Professor, PhD, Department of Beauty Science, Meiho Institute of technology, Taiwan, Pingtung County, Taiwan
  • Hung-Sheng Chi, Medical Doctor, Departemnt of Otolaryngology, Kaohsiung Medical University Hospital, Taiwan, Kaohsiung City, Taiwan
  • Kuen-Yao Ho, Associate Professor, Department of Otolaryngology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan

Main Contact E-mail: chenyu@kmu.edu.tw
Abstract:
Objectives: Clinical and audiologic factors known to predict treatment response and prognosis of sudden sensorineural hearing loss (SSNHL) have been reported as the time of presentation, the severity of hearing loss, and the presence of other factors such as vertigo and the slope of hearing loss.
However, studies examining specific biomarkers in blood (plasma and cells of buffy coat) and correlating to the clinical characteristics and prognosis of sudden sensorineural hearing loss are quite sparse. Proteomic technology has been recognized as a potent tool for identifying biomarkers by detecting the changes of proteins. This study is to employ proteomics for identifying disease biomarkers for sudden sensorineural hearing loss. Furthermore, the result may be used to predict the prognosis of patients with sudden sensorineural hearing loss.
Methods: A total of 76 patients were enrolled during the 20-month inclusion period. Human plasma and buffy coat were collected from 30 healthy people, 49 patients with sudden sensorineural hearing loss aged over 40 years, and 27 patients with sudden sensorineural hearing loss aged under 40 years. All patients have no history of diabetes mellitus (DM) and hypertension. After we removed the precipitate formed during dialysis by centrifugation. The recovered proteins of different samples were then precipitated by trichloride acid (TCA)/Acetone and used to run two dimensional electrophoresis (2DE) to establish the proteome maps of plasma and cells of buffy coat of healthy people and sudden sensorineural hearing loss patients. The protein spots were identified by liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) and they were compared among these three groups.
Results: Three differential proteins were identified by proteome map analysis in the cells of buffy coat of sudden sensorineural hearing loss patients, but not in the healthy people. The three proteins were lactoferrin, transferring and thioredoxin peroxidase B after liquid chromatography/mass spectrometry/ mass spectrometry (LC/MS/MS) analysis.
Conclusions: Thioredoxin peroxidase is the immediate enzyme that links reduction of H2O2 to thioredoxin. Several putative human thioredoxin peroxidases have been identified and involved in redox regulation of the cell. Thioredoxin peroxidase reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin and this may play an important role in eliminating peroxides generated during metabolism and participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. Thioredoxin peroxidase B is associated with the scavenging of free radicals. It is speculated that the occurrence of sudden sensorineural hearing loss is accompanied by oxidative damage to cause the increase of the level of thioredoxin peroxidase B. Further study of clinical hearing recovery will be carried out to evaluate if the above proteins are disease biomarkers for sudden sensorineural hearing loss.

Abstract Title: Indicators of Sudden Sensorineural Hearing Loss as Prognostic Factors For the Outcome
Main Organisation Work/Study Carried Out on Behalf of: University Clinic of ENT, Skopje, Republic of Macedonia
Main Authors:

  • Irena Duma-Vasovska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia

Additional Authors:

  • Marina Davceva Cakar, Professor, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Ilija Filipce, Professor, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Elena Bogeska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Valentina Ivanovska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia

Main Contact E-mail: irenadumavasovska@yahoo.com
Abstract:
Objective: Sudden sensorineural hearing loss  (SSNHL) is a disease whose main symptom represents a reduction of the ability to hear and is greater than 30 dB in at least three contiguous frequencies, that occur in a period of 72 hours or less. The purpose of the study is to test whether the outcome of SSNHL depends on degree of reduction of the ability to hear, the form of the audiometric curve, the presence of vertigo, and beginning of treatment.
Material and Methods: The study included 40 patients with SSNHL. The audiometric findings of affected ears were compared to contralateral, unaffected ear as a control. Each patient underwent PTA, tympanometry, Otoacoustic emissions, and caloric test.
Conclusion: Taking the tested factors into account which affect the outcome of SSNHL, using the multiple correlation analysis, we confirmed that the outcome of SSNHL depends on the presence of vertigo, the TEOAEs findings, the type of audiometric curve, and the time of therapy starting.

Abstract Title: The Role of Acellular Gelatinous Membrane in Vestibular Organs: Histopathlogical Study of Sudden Deafness With and Without Vertigo.
Main Authors:

  • Taro Inagaki, Researcher, Department of Otolaryngology, University of Minnesota, Minneapolis, USA

Additional Authors:

  • Sebahattin Cureoglu, Assistant Professor, Department of Otolaryngology, University of Minnesota, Minneapolis, USA
  • Mamoru Suzuki, Professor, Department of Otolaryngology, Tokyo Medical University, Tokyo, Japan
  • Michael M. Paparella, Professor, Department of Otolaryngology, University of Minnesota, Minneapolis, USA

Main Contact E-mail: inataro@tokyo-med.ac.jp
Abstract:
Objectives:  To investigate the histopathological difference between sudden deafness with vertigo (SDwV) and sudden deafness without vertigo (SDwoV) and the cause of persistent canal paresis (CP) in SDwV patients.
Methods:  Eight temporal bones from 4 unilateral sudden deafness (SD) patients, 2 SDwV and 2SDwoV, were selected. Four temporal bones of normal hearing ears were defined as control. Morphological findings of the labyrinth, the number of Scarpa's ganglion cells and the density of vestibular hair cells were investigated in all TBs. Clinical data of unilateral SD patients who performed a caloric test in the clinic were also investigated.
Results:  Atrophic change of the organ of Corti, tectorial membrame and stria vascularis in cochlea and deposits and atrophic otoconial membrane in the vestibular organs were observed on the affected ears. In one patient who died 10 months after the onset of sudden deafness, there were large amounts of deposits on the cupula. The otoconial membrane in saccular macula became thin and fell off, and the saccular space became narrow. There was no remarkable difference in the vestibular organ between SDwV and SDwoV. In the clinical data, all 4 SDwV patients who were examined within 2 years from the onset had caloric CP, and the patient who was examined more than 2 years after the onset had no CP. There were 3 patients with CP even in SDwoV. All SDwV patients had serious hearing loss.
Conclusion:  Loss of saccular hair cells seemed to occur easily in SD patients. There was no remarkable difference in histology between SDwV and SDwoV findings. We suspect that the atrophic change of acellular gelatinous membrane in the vestibular organ at the onset of hearing loss is irrespective of vestibular symptoms in SD patients, and a patient has vertigo when the damage is severe enough to cause the dysfunction of vestibular hair cells. Atrophic change of the cupula of lateral semicircular canal may cause persistent CP in SD patients.

 

Abstract Title: Mumps and Sudden Deafness
Main Organisation Work/Study Carried Out on Behalf of: University Clinic of ENT, Skopje, Republic of Macedonia
Main Authors:

  • Valentina Ivanovska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia

Additional Authors:

  • Marina Davceva-Cakar, Professor, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Ilija Filipce, Professor, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Elena Bogeska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia
  • Irena Duma Vasovska, Otorhinolaryngologist, University Clinic of ENT, Skopje, Republic of Macedonia

Main Contact E-mail: ivanvik@yahoo.com
Abstract:
Objectives: Mumps is one of the most common cause of unilateral sudden hearing loss and occasionally may be associated with vestibular symptoms.
Methods: During the epidemiology of mumps in the Republic of Macedonia this year there were 9 patients registered with unilateral sudden hearing loss. All patients underwent initial examination of the tympanic membrane, an audiogram, ENG examinations and laboratory evaluation.
Results: The results showed unilateral profound sensorineural hearing loss in all cases, hyporephlexio and arephlexio labyrinthi in two cases.
Conclusions: Hearing loss after mumps viremia is exclusively unilateral, severe or total and irreversible. An analysis of the present series of mumps deafness suggests that the primary route of invasion of the virus is hematogenous and thus proposed as the possible pathogenesis of deafness.

Abstract Title: Simultaneous Batroxobin, Betamethasone and Hyperbaric Oxygen Therapy for Idiopathic Sudden Sensorineural Hearing Loss
Main Organisation Work/Study Carried Out on Behalf of: Department of Otolaryngology, Osaka Rosai Hospital, Sakai, Japan
Main Authors:

  • Takefumi Kamakura, M.D. Department of Otolaryngology, Osaka Rosai Hospital, Sakai, Japan

Additional Authors:

  • Hiroshi Kajikawa, M.D.Ph.D. Department of Otolaryngology, Osaka Rosai Hospital, Sakai, Japan
  • Naoki Matsushiro, M.D.Ph.D. Department of Otolaryngology, Osaka Rosai Hospital, Sakai, Japan
  • Takahiro Kitamura, M.D. Department of Otolaryngology, Osaka Rosai Hospital, Sakai, Japan
  • Shin-ichi Okumura, M.D.Ph.D. Department of Otolaryngology, Osaka Rosai Hospital, Sakai, Japan

Main Contact E-mail: t-kamakura@orh.go.jp
Abstract:
Objectives: We should determine the efficacy of our therapy for patients with idiopathic sudden sensorineural hearing loss (ISSNHL) and the prognostic value of the severity and duration of hearing loss for the recovery from ISSNHL.
Methods: We investigated 474 patients with ISSNHL treated in Osaka Rosai Hospital from 2005 to 2008. The 474 patients received batroxobin, betamethasone and hyperbaric oxygen therapy simultaneously.
Results: Overall recovery rate was 34.4% in hearing. Patients with mild hearing loss at the onset showed a better recovery rate. Patients with short duration between the onset and the first visit showed a better recovery rate.
Conclusions: Our therapy for ISSNHL showed efficacious. Both the severity and duration of hearing loss had significant influence on the recovery rate from ISSNHL.